RNA purification using the RNeasy Mini Kit can be fully automated on the QIAcube Connect.
Read More: Oligotex mRNA Mini Kit. The QIAamp Viral RNA Mini Accessory Set provides the additional buffers and reagents needed for automated, low-throughput sample prep using both QIAcube and QIAamp Viral RNA Mini Kit.You are not authorized to download the resourceThe QIAamp Viral RNA Mini Kit simplifies purification of viral RNA from cell-free body fluids, offering fast spin-column or vacuum procedures, or automation on QIAcube. PCR inhibitors, such as divalent cations and proteins, are completely removed in two efficient wash steps, leaving pure RNA to be eluted in either water or a buffer provided with the kit.The QIAamp RNA Blood Mini Kit provides the highest-quality RNA with minimum copurification of DNA. QIAamp sample preparation technology is fully licensed.The QIAamp RNA Blood Mini Kit is designed for isolation of cellular RNA from up to 1.5 ml of fresh, whole human blood stabilized with any common anticoagulant, such as citrate, heparin, or EDTA. For certain RNA applications that are sensitive to very small amounts of DNA, it may be necessary to remove any remaining DNA. Rapid isolation of high-quality, ready-to-use RNA. For isolation of viral RNA from cell-free body fluids. For purification of up to 10 mg transfection-grade plasmid or cosmid DNA. For mRNA purification from total RNA and for in vitro transcript cleanup The QIAamp Viral RNA Mini Kit simplifies purification of viral RNA from cell-free body fluids with fast spin-column or vacuum procedures. No phenol–chloroform extraction is required.
In these cases, the QIAGEN RNase-Free DNase Set provides convenient on-column DNase treatment of RNA samples during QIAamp RNA procedures.QIAamp technology yields total cellular RNA from fresh whole blood and other sample sources that is ready to use in RT-PCR and blotting procedures.
No organic extraction or alcohol precipitation. Read More: QIAamp DNA Mini Kit. RNA binds specifically to the QIAamp silica-gel membrane while contaminants pass through.
Complete removal of contaminants and inhibitors. The QIAamp Viral RNA Mini Kit is automatable on the QIAcube. Consistent, high yields. Wash buffers are used to remove impurities and pure, ready-to-use RNA is then eluted in water or low-salt buffer. To guarantee RNA integrity, samples are lysed under highly denaturing conditions to inactivate RNases. After homogenization using the QIAshredder spin column, a fast spin-column procedure simplifies RNA purification. For smaller and larger samples, the RNeasy Micro Kit (spin-column binding capacity of up to 45 µg RNA), the RNeasy Midi Kit (spin-column binding capacity of 1 mg RNA), and RNeasy Maxi Kit (spin-column binding capacity of 6 mg RNA) are also available. Alcohol is added and lysates loaded onto the QIAamp spin column. In addition, total cellular RNA can be isolated from tissue samples.You are not authorized to download the resource The QIAamp RNA Blood Mini Kit provides purification of cellular RNA using silica-membrane technology. For rapid, easy, high-throughput mRNA purification from cells. The QIAamp RNA Blood Mini Kit provides silica-membrane-based purification of cellular RNA from up to 1.5 ml of fresh, whole human blood stabilized with any common anticoagulant, such as citrate, heparin, or EDTA. For purification of up to 100 µg total RNA from cells/tissues using gDNA Eliminator columns. RNA isolation exclusively from exosomes, exclusing non-vesicular protein complexes Convenient on-column procedure provides access to RNA in just one hour High sensitivity provided by maxi kit that allows large sample input volumes to detect low-abundance RNAs However, as with any RNA purification method, some DNA contamination can be expected. Read More: QIAGEN Plasmid Kits.
Sample types include:Optimized buffers and enzymes lyse samples, stabilize nucleic acids, and enhance selective RNA adsorption to the QIAamp membrane.
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qiagen rna extraction kit